Studies on Nuclear 4.5 S Ribonucleic Acid III of Novikoff Hepatoma Ascites Cells
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چکیده
منابع مشابه
Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.
The U-l RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus. After the RNA was purified by polyacrylamide gel electrophoresis, it was subjected to various enzymatic digestion procedures for determination of its primary nucleotide sequence. The U-l RNA contains 171 nucleotides. Its 5’ e...
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Evidence that 32 S nRNA contains 5.8 S rRNA was provided by studies on specific oligonucleotide sequences of these RNA species. Purified 32P-labeled 5.8 and 28 S rRNA and 32 S RNA were digested with T-1 ribonuclease, and the products were fractionated according to chain length by chromatography on DEAE-Sephadex A-25 at neutral pH. The oligonucleotides in Peak 8 were treated with alkaline phosph...
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Among the low molecular weight RNAs which have been shown to be localized to the nucleolus are the 4 S, U3, and 8 S RNAs. Of these, the U3 and 8 S RNAs are hydrogen bonded to nucleolar 28 S RNA. The nucleolar U3 RNA migrates as an electrophoretically homogeneous band on 8% polyacylamide gels but was separated into four bands on 16% gels. Although these bands have similar nucleotide compositions...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63012-1